The method of ascending preparative cell electrophoresis in a non-stabilized vertical column could be greatly improved by layering the cells on a starting cushion of buffer prepared in D2O and by stabilizing the liquid column above it with a D2O gradient. D2O/H2O mixtures have no apparent biochemical effects on the cells, and their physiochemical effects are shortlived and reversible. It was possible, by this method, to separate a mixture of glutaraldehyde-fixed erythrocytes of three species (rabbit, horse and chicken) into three distinct zones after 15 minutes of electrophoresis. (Kursetz)


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    Title :

    Preparative cell electrophoresis at 1 and 0 gravity


    Additional title:

    Vorbereitende Zellenelektrophorese bei einer Schwerkraft von 1 und 0


    Contributors:

    Published in:

    AIAA-Papers ; Jan ; 1-3


    Publication date :

    1979


    Size :

    3 Seiten, 3 Bilder, 1 Tabelle, 12 Quellen


    Type of media :

    Conference paper


    Type of material :

    Print


    Language :

    English