The method of ascending preparative cell electrophoresis in a non-stabilized vertical column could be greatly improved by layering the cells on a starting cushion of buffer prepared in D2O and by stabilizing the liquid column above it with a D2O gradient. D2O/H2O mixtures have no apparent biochemical effects on the cells, and their physiochemical effects are shortlived and reversible. It was possible, by this method, to separate a mixture of glutaraldehyde-fixed erythrocytes of three species (rabbit, horse and chicken) into three distinct zones after 15 minutes of electrophoresis. (Kursetz)
Preparative cell electrophoresis at 1 and 0 gravity
Vorbereitende Zellenelektrophorese bei einer Schwerkraft von 1 und 0
AIAA-Papers ; Jan ; 1-3
1979
3 Seiten, 3 Bilder, 1 Tabelle, 12 Quellen
Conference paper
English
Preparative cell electrophoresis at 1 and 0 gravity
AIAA | 1979
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