The method of ascending preparative cell electrophoresis in a non-stabilized vertical column could be greatly improved by layering the cells on a starting cushion of buffer prepared in D2O and by stabilizing the liquid column above it with a D2O gradient. D2O/H2O mixtures have no apparent biochemical effects on the cells, and their physiochemical effects are shortlived and reversible. It was possible, by this method, to separate a mixture of glutaraldehyde-fixed erythrocytes of three species (rabbit, horse and chicken) into three distinct zones after 15 minutes of electrophoresis. (Kursetz)


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    Titel :

    Preparative cell electrophoresis at 1 and 0 gravity


    Weitere Titelangaben:

    Vorbereitende Zellenelektrophorese bei einer Schwerkraft von 1 und 0


    Beteiligte:
    Oss, C.J.van (Autor:in) / Bronson, P.M. (Autor:in)

    Erschienen in:

    AIAA-Papers ; Jan ; 1-3


    Erscheinungsdatum :

    1979


    Format / Umfang :

    3 Seiten, 3 Bilder, 1 Tabelle, 12 Quellen


    Medientyp :

    Aufsatz (Konferenz)


    Format :

    Print


    Sprache :

    Englisch




    Preparative cell electrophoresis at 1 and 0 gravity

    VAN OSS, C. / BRONSON, P. | AIAA | 1979


    Preparative liquid column electrophoresis of T and B lymphocytes at gravity = 1

    VAN OSS, C. / BIGAZZI, P. / GILLMAN, C. et al. | AIAA | 1974



    Electrophoresis in space at zero gravity

    BIER, M. / SNYDER, R. | AIAA | 1974


    Kidney Cell Electrophoresis

    Todd, P. | NTRS | 1985