Single Loop for Cell Culture (SLCC) consists of individual, self-contained, spaceflight cell culture systems with capabilities for automated growth initiation, feeding, sub-culturing and sampling. The cells are grown and contained within a rigid cell specimen chamber (CSC). Bladder tanks provide flush and media fluid. SLCC uses active perfusion flow to provide nutrients and gas exchange, and to dilute waste products by expelling depleted media fluid into a waste bladder tank. The cells can be grown quiescently, or suspended using magnetically coupled stirrers. This paper describes the functional and safety design features, the operational modes and the spaceflight qualification processes including science validation tests, using yeast as a model organism.


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    Title :

    Single Loop for Cell Culture (SLCC) – Development and Spaceflight Qualification of a Perfusion Cell Culture System


    Additional title:

    Sae Technical Papers



    Conference:

    International Conference On Environmental Systems ; 2006



    Publication date :

    2006-07-17




    Type of media :

    Conference paper


    Type of material :

    Print


    Language :

    English




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