Single Loop for Cell Culture (SLCC) consists of individual, self-contained, spaceflight cell culture systems with capabilities for automated growth initiation, feeding, sub-culturing and sampling. The cells are grown and contained within a rigid cell specimen chamber (CSC). Bladder tanks provide flush and media fluid. SLCC uses active perfusion flow to provide nutrients and gas exchange, and to dilute waste products by expelling depleted media fluid into a waste bladder tank. The cells can be grown quiescently, or suspended using magnetically coupled stirrers. This paper describes the functional and safety design features, the operational modes and the spaceflight qualification processes including science validation tests, using yeast as a model organism.


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    Titel :

    Single Loop for Cell Culture (SLCC) – Development and Spaceflight Qualification of a Perfusion Cell Culture System


    Weitere Titelangaben:

    Sae Technical Papers


    Beteiligte:
    Hoehn, Alexander (Autor:in) / Vandendriesche, Donald (Autor:in) / Freeman, Jacob (Autor:in) / de Luis, Javier (Autor:in) / Parrish, Joe C. (Autor:in) / Sun, Liping (Autor:in) / Stodieck, Louis (Autor:in) / Vellone, Matthew (Autor:in) / Searby, Nancy D. (Autor:in) / Koenig, Paul (Autor:in)

    Kongress:

    International Conference On Environmental Systems ; 2006



    Erscheinungsdatum :

    17.07.2006




    Medientyp :

    Aufsatz (Konferenz)


    Format :

    Print


    Sprache :

    Englisch




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