Abstract In the present paper, we report on the effect of pH and carbonate on the hydrolysis rate constants of N-blocked and free aminoacyl adenylate anhydrides. Whereas the hydrolysis of free aminoacyly adenylates seems principally catalyzed by $ OH^{−} $, the hydrolysis of the N-blocked species is also catalyzed by $ H^{+} $, giving this compound a U-shaped hydrolysis vs. pH curve. Furthermore, at pH's<8, carbonate has an extreme catalytic effect on the hydrolysis of free aminoacyl-AMP anhydride, but essentially no effect on the hydrolysis of N-blocked aminoacyl-AMP anhydride. Furthermore, the N-blocked aminoacyl-AMP anhydride is a very efficient generator of peptides using free glycine as acceptor. The possible significance of the observations to prebiological peptide synthesis is discussed.


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    Title :

    Aminoacyl-nucleotide reactions: Studies related to the origin of the genetic code and protein synthesis


    Contributors:

    Published in:

    Origins of life ; 14 , 1-4 ; 597-604


    Publication date :

    1984




    Type of media :

    Article (Journal)


    Type of material :

    Print


    Language :

    English



    Classification :

    BKL:    44.71 Verkehrsmedizin
    Local classification TIB:    535/3908/4290





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