Abstract In the present paper, we report on the effect of pH and carbonate on the hydrolysis rate constants of N-blocked and free aminoacyl adenylate anhydrides. Whereas the hydrolysis of free aminoacyly adenylates seems principally catalyzed by $ OH^{−} $, the hydrolysis of the N-blocked species is also catalyzed by $ H^{+} $, giving this compound a U-shaped hydrolysis vs. pH curve. Furthermore, at pH's<8, carbonate has an extreme catalytic effect on the hydrolysis of free aminoacyl-AMP anhydride, but essentially no effect on the hydrolysis of N-blocked aminoacyl-AMP anhydride. Furthermore, the N-blocked aminoacyl-AMP anhydride is a very efficient generator of peptides using free glycine as acceptor. The possible significance of the observations to prebiological peptide synthesis is discussed.


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    Titel :

    Aminoacyl-nucleotide reactions: Studies related to the origin of the genetic code and protein synthesis


    Beteiligte:
    Mullins, D. W. (Autor:in) / Senaratne, N. (Autor:in) / Lacey, J. C. (Autor:in)

    Erschienen in:

    Erscheinungsdatum :

    1984




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Print


    Sprache :

    Englisch



    Klassifikation :

    Lokalklassifikation TIB:    535/3908/4290
    BKL:    44.71 Verkehrsmedizin