In this lecture, principle of fundamentally breaking the diffraction barrier through reversible saturable optical (fluorescence) transitions (RESOLFT) is discussed. In these concepts, the diffraction barrier is broken by a saturated optical transition (depletion) between two states of a marker, whereby the transition is effected with an intensity distribution featuring one or more intensity minima (zero). The saturation level defines the size of the ultrasharp focal spot and/or the concomitantly enlarged bandwidth of the optical transfer function (OTF).


    Access

    Check access

    Check availability in my library

    Order at Subito €


    Export, share and cite



    Title :

    Fluorescence nanoscopy: breaking the diffraction barrier by the RESOLFT concept


    Contributors:
    Hell, S.W. (author)


    Publication date :

    2005-01-01


    Size :

    129712 byte




    Type of media :

    Conference paper


    Type of material :

    Electronic Resource


    Language :

    English



    Fluorescence Nanoscopy: Breaking the Diffraction Barrier by the RESOLFT Concept

    IEEE Lasers and Electro-optics Society | British Library Conference Proceedings | 2005


    Breaking the Highway Capacity Barrier: Adaptive Cruise Control-Based Concept

    Godbole, Datta N. / Kourjanskaia, Natalia / Sengupta, Raja et al. | Transportation Research Record | 1999



    Shipborne underwater barrier breaking equipment and barrier breaking method

    NIE BAISHENG / JANG CHUL-HO / LIU XIANFENG et al. | European Patent Office | 2022

    Free access

    Breaking the barrier

    van Pelt, Michel | Springer Verlag | 2012