In this lecture, principle of fundamentally breaking the diffraction barrier through reversible saturable optical (fluorescence) transitions (RESOLFT) is discussed. In these concepts, the diffraction barrier is broken by a saturated optical transition (depletion) between two states of a marker, whereby the transition is effected with an intensity distribution featuring one or more intensity minima (zero). The saturation level defines the size of the ultrasharp focal spot and/or the concomitantly enlarged bandwidth of the optical transfer function (OTF).


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    Titel :

    Fluorescence nanoscopy: breaking the diffraction barrier by the RESOLFT concept


    Beteiligte:
    Hell, S.W. (Autor:in)


    Erscheinungsdatum :

    01.01.2005


    Format / Umfang :

    129712 byte




    Medientyp :

    Aufsatz (Konferenz)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch



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