We have developed a multifocal diffractive optical fluorescence correlation spectroscopy system for parallel excitation and detection of single tetramethylrhodamine (TMR-4-dUTP) biomolecules in microarrays (Gene chips). Multifocal excitation was made possible through the use of a 2 /spl times/ 2 fan-out diffractive optical element (DOE or kinoform) with uniform intensity in all four foci. Results of parallel excitation and detection in a high-density microarray of circular wells (o 70 /spl mu/m), using the multifocal diffractive optical element designed 2 /spl times/ 2 fan-out, show single biomolecule (TMR-4-dUTP) sensitivity in all foci, simultaneously. Future drug screening or functional genome analysis can hereby profit from this high sensitivity, parallel, fluorescence detection principle in biochip microarrays.


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    Title :

    Parallel fluorescence detection of single biomolecules in microarrays using a diffractive optical designed 2/spl times/2 fan-out element


    Contributors:
    Blom, H. (author) / Johansson, M. (author) / Hedman, A.-S. (author) / Lundberg, L. (author) / Hanning, A. (author) / Hard, S. (author) / Rigler, R. (author)


    Publication date :

    2003-01-01


    Size :

    57141 byte




    Type of media :

    Conference paper


    Type of material :

    Electronic Resource


    Language :

    English



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