We have developed a multifocal diffractive optical fluorescence correlation spectroscopy system for parallel excitation and detection of single tetramethylrhodamine (TMR-4-dUTP) biomolecules in microarrays (Gene chips). Multifocal excitation was made possible through the use of a 2 /spl times/ 2 fan-out diffractive optical element (DOE or kinoform) with uniform intensity in all four foci. Results of parallel excitation and detection in a high-density microarray of circular wells (o 70 /spl mu/m), using the multifocal diffractive optical element designed 2 /spl times/ 2 fan-out, show single biomolecule (TMR-4-dUTP) sensitivity in all foci, simultaneously. Future drug screening or functional genome analysis can hereby profit from this high sensitivity, parallel, fluorescence detection principle in biochip microarrays.
Parallel fluorescence detection of single biomolecules in microarrays using a diffractive optical designed 2/spl times/2 fan-out element
01.01.2003
57141 byte
Aufsatz (Konferenz)
Elektronische Ressource
Englisch
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