Abstract Exposure of fungal conidia ( Aspergillus ochraceus ) or spores of Bacillus subtilis to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered water activity results in an increasing number of cross-links between DNA and proteins. In fungal conidia these cross-links are detected after selective iodination (125J) of the DNA-bound proteins followed by gel electrophoresis and subsequent autoradiography. Another approach is the labelling of DNA with 32p by means of nick translation and the detection of differences in the electrophoretic mobility of DNA before and after digestion with proteinase K of proteins bound to DNA.


    Access

    Check access

    Check availability in my library

    Order at Subito €


    Export, share and cite



    Title :

    Extreme dryness and DNA-protein cross-links


    Contributors:
    Bieger-Dose, A. (author) / Dose, K. (author) / Meffert, R. (author) / Mehler, M. (author) / Risi, S. (author)

    Published in:

    Advances in Space Research ; 12 , 4 ; 265-270


    Publication date :

    1991-01-01


    Size :

    6 pages




    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English