AbstractOur scope was to study the effects of on ground random positioning machine rotation on swine testicular cells in culture. Cells of the 2 n karyotype line, from trypsinized swine testes, were submitted to modeled microgravity ( μ G ) using a 3D RPM from 15min to 24h. The cultured cells were then fixed and submitted to immunohistochemistry using antibodies to steroid dehydrogenases, heat shock proteins and the sodium pump ( Na + / K + ATPase).The results revealed that, after 15min at modeled μ G , all the cells showed damages at cytoskeletal level. The immunoreactions for 3 β HSD , 17 β HSD and Na + / K + ATPase were almost abolished. After 24h of treatment, the presence of the enzymes was restored, and small heat shock proteins were strongly immunostainable. On the other hand, in the 1 × G cultures, the expression of HSPs was very weak.We conclude that modeled μ G by random positioning of cells affects testicular cells in culture for a short time, while the normal activity of the cells is restored after 24h.


    Access

    Check access

    Check availability in my library

    Order at Subito €


    Export, share and cite



    Title :

    Effect of conditions of three dimensional clinostating on testicular cell machinery


    Contributors:

    Published in:

    Acta Astronautica ; 60 , 4-7 ; 391-396


    Publication date :

    2006-01-01


    Size :

    6 pages




    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English





    Effect of conditions of three dimensional clinostating on testicular cell machinery

    Uva, B. M. / Strollo, F. / Ricci, F. et al. | British Library Conference Proceedings | 2007


    Environmental conditions in ships' machinery spaces

    McDonald, R. | Engineering Index Backfile | 1962



    Improvement of environmental conditions in ships' machinery spaces

    Davidson, S.A. | Engineering Index Backfile | 1951