Digital holographic microscopy provides the ability to observe throughout a volume that is large compared to its resolution element without the need to refocus through the volume. This capability enables simultaneous observations of large numbers of small objects within such a volume. We have constructed a microscope that can observe a volume 0.4 x 0.4 x 1.0 μm with submicrometer resolution for observation of microorganisms and minerals in liquid environments in earth and on potential planetary missions. Because environmental samples are likely to contain mixtures of inorganics and microorganisms that are of comparable sizes near the resolution limit of the instrument, discrimination of living and non-living objects may be difficult. The motion of motile organisms can be used to readily distinguish them from non-motile objects (live or inorganic), but additional methods are required to distinguish non-motile organisms and inorganic objects that are of comparable size but different composition and structure. In this paper we evaluate the use of passive motion to make this discrimination by evaluating diffusion and buoyancy characteristics of objects in the field of view.


    Access

    Check access

    Check availability in my library

    Order at Subito €


    Export, share and cite



    Title :

    Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy




    Publication date :

    2020-03-01


    Size :

    3362658 byte




    Type of media :

    Conference paper


    Type of material :

    Electronic Resource


    Language :

    English



    Microscopic urinalysis by digital holographic microscopy

    Rastogi, Vivek / Agarwal, Shilpi / Dubey, Satish Kumar et al. | British Library Conference Proceedings | 2019


    Holographic Microscopy Through Tubes And Ducts

    McGehee, J. H. / Wuerker, R. F. | SPIE | 1985


    Buried object classification using holographic radar

    Windsor, C. / Capineri, L. / Bechtel, T.D. | Tema Archive | 2012


    Holographic Microscopy

    Wuerker, R. F. / Hill, D. A. | SPIE | 1985


    Holographic Microscopy System

    Witherow, W. K. | NTRS | 1983