In this paper, we describe methods to increase the functionality of conventional optical tweezer techniques through the use of a 410 nm laser diode to induce fluorescence in biological samples. For the first time, to our knowledge, we demonstrate the use of a violet diode laser to induce fluorescence in particles, which are simultaneously tweezed by an infrared laser. Using this technique we demonstrate the sorting of fluorescent polymer spheres and DAPI-stained chromosomes.


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    Title :

    Simultaneous optical tweezing and laser-induced fluorescence of DAPI stained chromosomes


    Contributors:
    Carruthers, A.E. (author) / Lake, T.K. (author) / Paterson, L. (author) / Allen, J.W. (author) / Sibbett, W. (author) / Dholakia, K. (author)


    Publication date :

    2003-01-01


    Size :

    111860 byte




    Type of media :

    Conference paper


    Type of material :

    Electronic Resource


    Language :

    English



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