We demonstrate the first use of femtosecond laser pulses for serial histology. Successive iterations of multiphoton imaging and ablation provide diffraction-limited volumetric data that is used to reconstruct the architectonics of labeled cells or microvasculature.


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    Title :

    All optical histology of brain tissue: serial ablation and multiphoton imaging with femtosecond laser pulses


    Contributors:
    Tsai, P.S. (author) / Friedman, B. (author) / Lev-Ram, V. (author) / Xiong, Q. (author) / Tsien, R.Y. (author) / Kleinfeld, D. (author) / Ifarraguerri, A.I. (author) / Thompson, B.D. (author) / Squier, J.A. (author)


    Publication date :

    2003-01-01


    Size :

    476950 byte



    Type of media :

    Conference paper


    Type of material :

    Electronic Resource


    Language :

    English





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