Abstract Volatile organic compounds (VOCs) – biomarkers of oxidative stress in breath of 25 healthy volunteers were analyzed using gas chromatography – mass spectrometry in two series of experiments: 1- and 2-h of normobaric exposure to 100% medical oxygen while resting comfortably in seated position. Randomization of the breath study made it possible to determine VOCs responding to the oxygen exposure: ethane, isoprene, propane, 3-methylhexane, heptanone-2, 2-methylhexane, butane, isobutane, methylcyclopentane, 3-methylbutanal, 2-methylpentane. The biomarkers, which are typical for oxidative stress, were verified using discriminant analysis that allowed us to exclude ethane, isoprene, propane, 2-methylhexane, butane, isobutene and methylcyclopentane from the mathematical model due to the low information content for the forecasting model. Discrimination analysis was conducted in a stepwise mode, which is based on minimization of the Wilkes coefficient (λ) using standard algorithms of the Statistica Statsoft (version 8). Data discrimination allowed to develop a mathematical model and to establish four biomarkers of oxidative stress: 3-methylbutanal, heptanone-2, 3-methylhexane, 2-methylpentane. The predictive model was cross-validated. The forecast accuracy of the developed model is 70%.

    Highlights Exhaled air of a human has chemical biomarkers that respond to oxygen inhalation. Most rapidly developing methods of metabolic diagnostics. Non-invasive express studies of human's functional state in real time. Physiological adaptation to spaceflight conditions.


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    Title :

    Molecular metabolites – biomarkers of oxidative stress in healthy human breath during normobaric hyperoxygenation


    Contributors:
    Moukhamedieva, L. (author) / Dolch, M. (author) / Ozerov, D. (author) / Fetter, V. (author) / Lenic, J. (author) / Kornienko, A. (author) / Grosser, J. (author) / Tsarkov, D. (author) / Roth, P. (author) / Nitzer, R. (author)

    Published in:

    Acta Astronautica ; 199 ; 456-463


    Publication date :

    2022-05-31


    Size :

    8 pages




    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English




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