AbstractThe objective of this study was to measure differences in the chromosomal rearrangements found in the progeny of cells exposed to the same dose of X-rays or energetic iron ions, with emphasis on intra-chromosomal exchanges. We hybridized metaphase cells with human DNA probes specific for the p and q arms of the chromosome 1. The arm-specific probes allow a fast and reliable detection of both symmetrical and asymmetrical inter-chromosomal exchanges and inter-arm intra-changes (pericentric inversions) in the painted chromosome pair. We used this method to score aberrations in human peripheral blood lymphocytes exposed to 1Gy of either 250kVp X-rays or 1GeV/n Fe-ions and harvested following 120h in culture, including 2h in colcemid for metaphase-block. Although iron ions are much more effective than X-rays in the induction of chromosomal aberrations formed during the first post-exposure cell cycle, we found that the effectiveness drops when daughter cells are scored at a late harvest time. In fact, no significant difference in the yield of simple interchanges or intra-changes was found for the two radiation qualities. On the other hand, complex-type exchanges, including rearrangements involving both intra- and inter-chromosomal exchanges, were much more frequent in the progeny of the population exposed to Fe-nuclei than to photons.


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    Title :

    Rearrangements in human chromosome 1 visualized by arm-specific probes in the progeny of blood lymphocytes exposed to iron ions


    Contributors:
    Manti, L. (author) / Bertucci, A. (author) / Gialanella, G. (author) / Grossi, G. (author) / Pignalosa, D. (author) / Pugliese, M. (author) / Scampoli, P. (author) / Durante, M. (author)

    Published in:

    Advances in Space Research ; 39 , 6 ; 1066-1069


    Publication date :

    2006-11-15


    Size :

    4 pages




    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English