AbstractRecent studies have demonstrated that stem cells derived from adult hematopoietic tissues are capable of trans-differentiation into non-hematopoietic cells, and that the culture in microgravity (g) may modulate the proliferation and differentiation. We investigated the application of g to human umbilical cord blood stem cells (CBSC) in the induction of vascular endothelial phenotype expression and cellular proliferation.CD34+ mononuclear cells were isolated from waste human umbilical cord blood samples and cultured in simulated g for 14 days. The cells were seeded in rotary wall vessels (RWV) with or without microcarrier beads (MCB) and vascular endothelial growth factor was added during culture. Controls consisted of culture in 1G. The cell cultures in RWV were examined by inverted microscopy. Cell counts, endothelial cell and leukocyte markers performed by flow-cytometry and FACS scan were assayed at days 1, 4, 7 and at the termination of the experiments. Culture in RWV revealed significantly increased cellular proliferation with three-dimensional (3D) tissue-like aggregates. At day 4, CD34+ cells cultured in RWV bioreactor without MCB developed vascular tubular assemblies and exhibited endothelial phenotypic markers.These data suggest that CD34+ human umbilical cord blood progenitors are capable of trans-differentiation into vascular endothelial cell phenotype and assemble into 3D tissue structures. Culture of CBSC in simulated g may be potentially beneficial in the fields of stem cell biology and somatic cell therapy.
Induction of vascular endothelial phenotype and cellular proliferation from human cord blood stem cells cultured in simulated microgravity
Acta Astronautica ; 56 , 9-12 ; 918-922
2005-01-01
5 pages
Article (Journal)
Electronic Resource
English
British Library Conference Proceedings | 2005
|British Library Conference Proceedings | 2005
|Effects of simulated microgravity on cell cycle in human endothelial cells
Online Contents | 2014
|