AbstractThe nematode Caenorhabditis elegans was exposed to natural space radiation using the ESA Biorack facility aboard Spacelab on International Microgravity Laboratory 1, STS-42. For the major experimental objective dormant animals were suspended in buffer or on agar or immobilized next to CR-39 plastic nuclear track detectors to correlate fluence of HZE particles with genetic events. This configuration was used to isolate mutations in a set of 350 essential genes as well as in the unc-22 structural gene. From flight samples 13 mutants in the unc-22 gene were isolated along with 53 lethal mutations from autosomal regions balanced by a translocation eT1 (III;V). Preliminary analysis suggests that mutants from worms correlated with specific cosmic ray tracks may have a higher proportion of rearrangements than those isolated from tube cultures on a randomly sampled basis. Flight sample mutation rate was approximately 8-fold higher than ground controls which exhibited laboratory spontaneous frequencies.


    Access

    Check access

    Check availability in my library

    Order at Subito €


    Export, share and cite



    Title :

    Radiation effects in nematodes: Results from IML-1 experiments


    Contributors:
    Nelson, G.A. (author) / Schubert, W.W. (author) / Kazarians, G.A. (author) / Richards, G.F. (author) / Benton, E.V. (author) / Benton, E.R. (author) / Henke, R. (author)

    Published in:

    Publication date :

    1994-01-01


    Size :

    5 pages




    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English




    Radiation effects in nematodes: Results from IML-1 experiments

    Nelson, G. A. / Schubert, W. W. / Kazarians, G. A. et al. | NTRS | 1994


    Development and chromosome mechanics in nematodes: Results from IML-1

    Nelson, G.A. / Schubert, W.W. / Kazarians, G.A. et al. | Elsevier | 1994


    Development and Chromosome Mechanics in Nematodes: Results from IML-1

    Nelson, G. A. / Schubert, W. W. / Kazarians, G. A. et al. | British Library Conference Proceedings | 1994


    Development and chromosome mechanics in nematodes: Results from IML-1

    Nelson, G. A. / Schubert, W. W. / Kazarians, G. A. et al. | NTRS | 1994