AbstractThe aim of this study was to estimate the acute effects of low dose 12C6+ ions or X-ray radiation on human immune function. The human peripheral blood lymphocytes (HPBL) of seven healthy donors were exposed to 0.05Gy 12C6+ ions or X-ray radiation and cell responses were measured at 24h after exposure. The cytotoxic activities of HPBL were determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT); the percentages of T and NK cells subsets were detected by flow cytometry; mRNA expression of interleukin (IL)-2, tumor necrosis factor (TNF)-α and interferon (IFN)-γ were examined by real time quantitative RT-PCR (qRT-PCR); and these cytokines protein levels in supernatant of cultured cells were assayed by enzyme-linked immunosorbent assays (ELISA). The results showed that the cytotoxic activity of HPBL, mRNA expression of IL-2, IFN-γ and TNF-α in HPBL and their protein levels in supernatant were significantly increased at 24h after exposure to 0.05Gy 12C6+ ions radiation and the effects were stronger than observed for X-ray exposure. However, there was no significant change in the percentage of T and NK cells subsets of HPBL. These results suggested that 0.05Gy high linear energy transfer (LET) 12C6+ radiation was a more effective approach to host immune enhancement than that of low LET X-ray. We conclude that cytokines production might be used as sensitive indicators of acute response to LDI.


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    Title :

    Early effects of low dose 12C6+ ion or X-ray irradiation on human peripheral blood lymphocytes


    Contributors:
    Chen, Yingtai (author) / Li, Yumin (author) / Zhang, Hong (author) / Xie, Yi (author) / Chen, Xuezhong (author) / Ren, Jinyu (author) / Zhang, Xiaowei (author) / Zhu, Zijiang (author) / Liu, Hongliang (author) / Zhang, Yawei (author)

    Published in:

    Advances in Space Research ; 45 , 7 ; 832-838


    Publication date :

    2009-09-30


    Size :

    7 pages




    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English