AbstractThe aims of this study are to investigate the effects of gravitational environment produced by a superconducting magnet on osteoblast morphology, proliferation and adhesion. A superconducting magnet which can produce large gradient high magnetic field (LGHMF) and provide three apparent gravity levels ( 0 g , 1 g and 2 g ) was employed to simulate space gravity environment. The effects of LGHMF on osteoblast morphology, proliferation, adhesion and the gene expression of fibronectin and collagen I were detected by scanning electron microscopy, immunocytochemistry, adhesion assays and real time PCR, respectively, after exposure of osteoblasts to LGHMF for 24h. Osteoblast morphology was affected by LGHMF ( 0 g , 1 g and 2 g ) and the most evident morphology alteration was observed at 0 g condition. Proliferative abilities of MC3T3 and MG-63 cell were affected under LGHMF ( 0 g , 1 g and 2 g ) conditions compared to control condition. The adhesive abilities of MC3T3 and MG-63 cells to extracellular matrix (ECM) proteins (fibronectin, laminin, collagen IV) were also affected by LGHMF ( 0 g , 1 g and 2 g ) , moreover, the effects of LGHMF on osteoblast adhesion to different ECM proteins were different. Fibronectin gene expression in MG63 cells under zero gravity condition was increased significantly compared to other conditions. Collagen I gene expression in MG-63 and MC3T3 cells was altered by both magnetic field and alerted gravity. The study indicates that the superconducting magnet which can produce LGHMF may be a novel ground-based space gravity simulator and can be used for biological experiment at cellular level.


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    Title :

    Gravitational environment produced by a superconducting magnet affects osteoblast morphology and functions


    Contributors:
    Qian, Airong (author) / Zhang, Wei (author) / Weng, Yuanyuan (author) / Tian, Zongcheng (author) / Di, Shengmeng (author) / Yang, Pengfei (author) / Yin, Dachuan (author) / Hu, Lifang (author) / Wang, Zhe (author) / Xu, Huiyun (author)

    Published in:

    Acta Astronautica ; 63 , 7-10 ; 929-946


    Publication date :

    2008-03-02


    Size :

    18 pages




    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English





    Gravitational environment produced by a superconducting magnet affects osteoblast morphology and functions

    Qian, A. / Zhang, W. / Weng, Y. et al. | British Library Conference Proceedings | 2008



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