Alginate-based hydrogels are being widely used in tissue engineering due to their biocompatible character. However, alginate hydrogels degrade very slowly and exhibit poor cell adhesion capability because of their lack of specific biomolecular anchoring sites for mammalian cells. The oxidation of alginate leading to alginate dialdehyde (ADA) can enhance its biodegradability. The incorporation of specific proteins such as gelatin into alginate based hydrogels can improve the cell-matrix interaction of the hydrogel, gelatin, for example, contains the cell adhesive peptide sequence, namely RGD (arginine-glycine-aspartic acid). In this research project, alginate dialdehyde-gelatin (ADA-GEL) based hydrogels were utilized for scaffold development and biofabrication for tissue engineering applications. The main goal was to develop new technologies to expand the applications of ADA-GEL in a variety of form. Porous ADA-GEL scaffolds were produced via freeze-drying process as a standard scaffold fabrication method. The scaffolds were coated with mesoporous bioactive glass nanoparticles (MBGN) using dip coating method. The compressive strength and elastic modulus of the scaffolds coated with MBGN were higher than that of non-coated samples but the difference was not significant. The coating process did not change the microstructure of the scaffolds. The presence of MBGN diminished the degradability of the samples. The formation of HA layer on the scaffolds coated with MBGN was observed, indicating that MBGN coating could enhance the bioactivity of ADA-GEL based freeze-dried scaffolds, intended for bone tissue engineering. The viability of MCT3T-E1 cells cultured on uncoated scaffolds was higher than that on the MBGN-coated samples over 21 days of cultivation. Nevertheless, the positive effect of MBGN on osteogenic differentiation of MCT3T-E1 cells was observed. A second technology investigated involved the development of mineralizing ADA-GEL composite microcapsules. Composite microcapsules based on ADA, gelatin, and 45S5 ...
Alginate-Gelatin Based Hydrogels for Scaffold Development and Biofabrication in Tissue Engineering
2021-01-01
Theses
Electronic Resource
English
DDC: | 629 |
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