This study presents an integrated microfluidic system capable of automatically performing four reverse-transcription polymerase chain reaction (RT-PCR) processes simultaneously for fast diagnosis of aquacultural diseases. This system integrates micro temperature control modules and a microfluidic control module. The micro temperature control modules have micro temperature sensors and array-type micro heaters that maintain precise and uniform temperature conditions for the RT-PCR processes. The microfluidic control module can automatically transport samples and reagents by using pneumatic micropumps, microvalves and microchannels. Moreover, by using random primers in the reverse-transcription (RT) process, the chip design is simplified and the consumption of RT products in the subsequent multiple polymerase chain reactions (PCR) is also minimized. After sample transport between the RT chamber and the PCR chambers is finished, the PCR process is then performed to amplify detection genes for each ribonucleic acid (RNA)-based virus. This microfluidic chip system, fabricated by using micro-electro-mechanical-system (MEMS) technology, has the following advantages, including high sensitivity, fast diagnosis, disposability, low reagent and sample consumption, portability, and low power consumption. Experimental data show that the developed system can successfully detect four types of purified RNA samples, including the nervous necrosis virus (NNV), Iridovirus, Vibrio anguillarum and the grouper Mx protein gene. The detection limit of the developed system is found to be 10 copies/microlitre, which is better than the limits of conventional methods which are approximately 100-1000 copies/microlitre. The total reaction time for the detection of the four disease markers is experimentally found to be about 2.5 h. Consequently, the developed microfluidic system may provide a powerful tool for fast diagnosis of RNA-based viral vectors which cause aquaculture diseases.


    Access

    Access via TIB

    Check availability in my library

    Order at Subito €


    Export, share and cite



    Title :

    A microfluidic-based system using reverse transcription polymerase chain reactions for rapid detection of aquaculture diseases


    Additional title:

    Ein mikrofluidisch-basiertes System für reverse Transkriptase-Polymerase-Kettenreaktionen zum Nachweis von Aquakultur-Krankheiten


    Contributors:

    Published in:

    Publication date :

    2009


    Size :

    12 Seiten, 9 Bilder, 30 Quellen




    Type of media :

    Article (Journal)


    Type of material :

    Print


    Language :

    English




    High-throughput PCR (Polymerase Chain Reaction) detection laboratory

    WANG RONGXIN / LI ZHAOBING / HONG FENGTAO et al. | European Patent Office | 2023

    Free access

    Distributed detection system for aquaculture water body

    MA HAIQIN / MA YONGXIN / LU LINFA et al. | European Patent Office | 2023

    Free access


    Reverse bionic mechanical turtle for detecting water area environment in aquaculture industry

    SHI YAN / ZHAO LIAN / YUE XIANGCHENG et al. | European Patent Office | 2023

    Free access

    REVERSE RUNNING DETECTION DEVICE, REVERSE RUNNING DETECTION SYSTEM, AND REVERSE RUNNING DETECTION METHOD

    KANEKO SHIGEKI / SUGITANI TAKESHI | European Patent Office | 2018

    Free access