Two oscillator circuits capable of oscillating an AT cut piezoelectric crystal in contact with liquid were built and tested. E.coli concentrations in the range of 700 cells/ml can be detected using the total immersion techniques. Scanning electron microscope images revealed that ‘under-liquid’ biosensors might offer lower detection limits than the dip-and-dry method. The effect of water temperature on the crystal frequency was found to be linear in the range of 18 - 45 °C. A linear decrease in crystal frequency was noticed as the relative viscosity increased from 1 to 3. These oscillator circuits were duplicated and integrated to result in a Computer Automated Biosensor Array (CABA), which was designed to overcome the antibody selectivity problems. This system utilizes a commercially available pattern recognition tool to fingerprint a particular antigen. The proposed technology will enhance abilities in fingerprinting of antigens and alleviate some of the problems associated with cross reactivity.


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    Title :

    A Piezoelectric Crystal Immunosensor for E.coli


    Additional title:

    Sae Technical Papers


    Contributors:

    Conference:

    International Conference On Environmental Systems ; 1997



    Publication date :

    1997-07-01




    Type of media :

    Conference paper


    Type of material :

    Print


    Language :

    English




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