Highlights G0 and G1 phase MRC5 cells were prepared and treated by different HZE particles. Cell survival and DNA DSBs damage levels were measured to evaluate radiation toxicity. All kinds of particles were dose-dependently lethal through colony assay. 53BP1 foci were induced in a LET- and fluence-dependent manner. G0 cells have higher survival levels and less DSBs than G1 cells.

    Abstract High charge and energy (HZE) particles are severe risk to manned long-term outer space exploration. Studies on the biological effects of space HZE particles and the underlying mechanisms are essential to the accurate risk assessment and the development of efficient countermeasure. Since majority of the cells in human body stay quiescent (G0 phase), in this study, we established G0 cell and G1 cell models by releasing human normal embryonic lung fibroblast cells from contact inhibition and studied the radiation toxicity of various kinds of HZE particles. Results showed that all of the particles were dose-dependently lethal and G0 cells were more radioresistant than G1 cells. We also found that 53BP1 foci were induced in a LET- and fluence-dependent manner and fewer foci were induced in G0 cells than G1 cells, however, the decrease of foci in 24h after irradiation was highly relevant to the type of particles. These results imply that even though health risk of space radiation is probably overestimated by the data obtained with exponentially growing cells, whose radiosensitivity is similar to G1 cells, the risk of space HZE particles is un-ignorable and accurate assessment and mechanistic studies should be deepened. The diverse abilities of G0 cells and G1 cells in repairing DNA damages induced by HZE particles emphasize the importance in studying the impact of HZE particles on DNA damage repair pathways.


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    Title :

    Simulated studies on the biological effects of space radiation on quiescent human fibroblasts


    Contributors:
    Ding, Nan (author) / Pei, Hailong (author) / He, Jinpeng (author) / Furusawa, Yoshiya (author) / Hirayama, Ryoichi (author) / Liu, Cuihua (author) / Matsumoto, Yoshitaka (author) / Li, He (author) / Hu, Wentao (author) / Li, Yinghui (author)

    Published in:

    Advances in Space Research ; 52 , 7 ; 1314-1319


    Publication date :

    2013-06-28


    Size :

    6 pages




    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English