Abstract Online pH control during microbial shake flask cultivation has not been established due to the lack of a practical combination of an online sensor system and an appropriate control unit. The objective of this investigation was to develop a minimum scale dosage apparatus, namely shake flask controller ("SFC"), which can control the pH during a complete cultivation and serves as technical example for the application of small liquid dispensing lab devices. A well evaluated optical, chemosensor based, noninvasive, multisensory platform prototype for online DO (dissolved oxygen)-, pH- and biomass measurement served as sensor. The SFC was designed as cap-integrated, semi-autarkical control unit. Minimum scale working parts like the commercial mp6 piezoelectric micropumps and miniature solenoid valves were combined with a selective laser sintering (SLS) printed backbone. In general it is intended to extend its application range on the control of enzymatic assays, polymerization processes, cell disruption methods or the precise dispense of special chemicals like inducers or inhibitors. It could be proved that pH control within a range of 0.1 pH units could be maintained at different cultivation conditions. A proportional-integral-derivative- (PID) controller and an adaptive proportional controller were successfully applied to calculate the balancing solution volume. SLS based 3D printing using polyamide combined with state-of-the-art micro pumps proved to be perfectly adaptable for minimum size, autoclavable lab devices. ; BMWi


    Access

    Download


    Export, share and cite



    Title :

    New perspectives in shake flask pH control using a 3D-printed control unit based on pH online measurement


    Contributors:

    Publication date :

    2015-01-01


    Remarks:

    Sensors and Actuators, B: Chemical 221 (2015)



    Type of media :

    Article (Journal)


    Type of material :

    Electronic Resource


    Language :

    English



    Classification :



    Big flask

    Engineering Index Backfile | 1954


    CRANE SHAKE MEASUREMENT DEVICE, SHAKE STOPPING DEVICE, AND SHAKE STOPPING METHOD

    TAGAWA YASUYOSHI / SUZUKI HIROYUKI / IWAMA YUICHI et al. | European Patent Office | 2021

    Free access

    SHAKE CONTROL ON CONVERTIBLES

    KAMINS, M. / LOVE, W. B. | SAE Technical Papers | 1954


    Airplane control surface anti-shake measurement system and method

    GAO HONGWEI / YANG TIANYU / LIU GUANZHANG et al. | European Patent Office | 2022

    Free access

    Anti-shake control method and control system of vehicle

    CHO WOO CHEOL | European Patent Office | 2023

    Free access