We present images of human skin obtained by a two-photon fluorescence microscope, which has been optimized for in- vivo imaging. Using autofluorescence as a contrast mechanism various layers of the skin, e.g., stratum corneum, viable epidermis, basal layer and upper dermis could be clearly distinguished. A comparison between two-photon fluorescence images and reflectance images shows that there is a clear difference in the information content provided by both techniques. In particular, the fluorescence images of the dermis show detail that is absent in the reflectance images.


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    Titel :

    Two-photon fluorescence microscopy of in-vivo human skin


    Beteiligte:

    Kongress:

    Laser Microscopy ; 2000 ; Amsterdam,Netherlands


    Erschienen in:

    Erscheinungsdatum :

    2000-12-18





    Medientyp :

    Aufsatz (Konferenz)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch





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