Organotypic cultures of precision-cut rat lung slices are exposed three hours to 5 dilutions of a continuous exhaust flow. The exhausts from three fuelings are tested (filtered and not): gazole, gazole/RME mixture (70 % / 30%) and RME.Different parameters are assessed: ATP and GSH (cell viability), TNFα production (proinflammatory response) and nucleosomes (apoptosis). After exposure, the ATP level is only modified by the highest concentrations of gazole/RME or RME exhausts (filtered and not); the GSH level is decreased for each of the concentrated filtered exhausts. Moreover, only the whole gazole exhaust leads to a modification of TNFα production. At last, the whole gazole exhaust leads to an increase of slice nucleosome level in a dose-dependent way; RME supplementation or filtration significantly attenuates this effect.In conclusion, whole diesel exhaust induces an inflammatory response and DNA alterations, which are partially or totally reversed by filtration and/or by RME supplementation.


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    Titel :

    Toxicological Impact of Diesel Fuel Supplementation with Rapeseed Methylester (RME) on the Lung Toxic Potential of Diesel Engine Exhausts


    Weitere Titelangaben:

    Sae Technical Papers


    Beteiligte:
    Bion, Anne (Autor:in) / Le Prieur, Elsa (Autor:in) / Gouriou, Frantz (Autor:in) / Dionnet, Frédéric (Autor:in) / Morin, Jean Paul (Autor:in)

    Kongress:

    CEC/SAE Spring Fuels & Lubricants Meeting & Exposition ; 2000



    Erscheinungsdatum :

    2000-06-19




    Medientyp :

    Aufsatz (Konferenz)


    Format :

    Print


    Sprache :

    Englisch





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