Introduction: Routine assessment of the International Space Station (ISS) microbiome has been performed through in-flight culture and Earth-based analysis. Through utilization of the ISS, significant progress toward in situ microbial identification has been achieved. In 2016, the molecular space age began when DNA-based, Earth-prepared samples were amplified within miniPCR (Genes in Space-1), and subsequent samples were sequenced with the MinION (Biomolecule Sequencer). The following year, these platforms were used collectively to yield the first off-Earth identification of unknown bacteria collected and cultured onboard the ISS (Genes in Space-3). Further advancement occurred in 2018 when a culture-independent, direct swab-to-sequencer method revealed a more thorough depiction of the ISS surface microbiome (BEST). As NASA looks towards Artemis and extended exploration missions, it is critical to continue to harness the ISS to expand in situ nanopore sequencing-based analysis in support of microbial-related crew health, planetary protection, and space research initiatives.


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    Titel :

    Nanopore Sequencing in Space: The Advancement of In Situ Microbiome Analysis for the International Space Station and Beyond


    Beteiligte:
    Christian G. Mena (Autor:in) / Sarah Stahl-Rommel (Autor:in) / Janejit T. Gensler (Autor:in) / Hang N. Nguyen (Autor:in) / G. Marie Sharp (Autor:in) / Mark R. Fernandez (Autor:in) / Naeem Altaf (Autor:in) / Minsik Lee (Autor:in) / Miten Jain (Autor:in) / Sarah L. Castro-Wallace (Autor:in)

    Kongress:

    12th Annual International Space Station Research and Development Conference (ISSRDC) ; 2023 ; Seattle, WA, US


    Medientyp :

    Sonstige


    Format :

    Keine Angabe


    Sprache :

    Englisch


    Schlagwörter :


    The BioMole Facility: Advancement of In Situ Microbiome Analysis for the International Space Station

    Sarah Wallace / Sarah Stahl-Rommel / Janejit Tanghongs Gensler et al. | NTRS


    Beyond Nanopore Sequencing in Space: Identifying the Unknown

    Stahl, Sarah E. / Burton, Aaron S. / John, Kristen K. et al. | NTRS | 2017



    Unlocking the Microbiome of the International Space Station

    Sarah Stahl-Rommel / Hang N. Nguyen / G. Marie Sharp et al. | NTRS