Microgravity has advantages for the cultivation of tissues with high fidelity; however, tissue formation requires cellular recognition and adhesion. We tested the hypothesis that simulated microgravity does not affect cell adhesion. Human colorectal carcinoma cells were cultured in the NASA Rotating Wall Vessel (RWV) under low shear stress with randomization of the gravity vector that simulates microgravity. After 6 - 7 days, cells were assayed for binding to various substrates and compared to cells grown in standard tissue culture flasks and static suspension cultures. The RWV cultures bound as well to basement membrane proteins and to Carcinoembryonic Antigen (CEA), an intercellular adhesion molecule, as control cultures did. Thus, microgravity does not alter epithelial cell adhesion and may be useful for tissue engineering.


    Zugriff

    Zugriff über TIB

    Verfügbarkeit in meiner Bibliothek prüfen


    Exportieren, teilen und zitieren



    Titel :

    Simulated microgravity does not alter epithelial cell adhesion to matrix and other molecules


    Beteiligte:
    Jessup, J. M. (Autor:in) / Brown, K. (Autor:in) / Ishii, S. (Autor:in) / Ford, R. (Autor:in) / Goodwin, T. J. (Autor:in) / Spaulding, G. (Autor:in)


    Erscheinungsdatum :

    1994-08-01




    Medientyp :

    Sonstige


    Format :

    Keine Angabe


    Sprache :

    Englisch




    Simulated Microgravity does not Alter Epithelial Cell Adhesion to Matrix and Other Molecules

    Jessup, J. M. / Brown, K. / Ishii, S. et al. | British Library Conference Proceedings | 1994



    Epithelial adhesion molecules in airway repair

    Spurzem, J. R. | British Library Conference Proceedings | 1997


    Simulated microgravity impairs vascular contractility

    Purdy, R. E. / Sara, D. / Duckles, S. P. et al. | NTRS | 1997


    In vitro effects of simulated microgravity on Sertoli cell function

    Masini, M.A. / Prato, P. / Scarabelli, L. et al. | Elsevier | 2010