We propose a technique for improving the image quality in fluorescence confocal microscopy imaging by exploiting the phase difference of the fluorescence emission from different depths of the sample. The technique uses a modulated excitation beam and homodyne detection scheme. The image after homodyne detection consists of an unmodulated term and a sinusoidally modulated term. The unmodulated term does not have any phase dependence. The modulated term has a phase dependence on the depth of the sample from which emission is occurring. In the proposed methodology, the unmodulated term is removed giving rise to images with better depth perception.
Improvement in fluorescence confocal microscopy for obtaining better depth perception
2008-12-01
367726 byte
Aufsatz (Konferenz)
Elektronische Ressource
Englisch
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