We propose a technique for improving the image quality in fluorescence confocal microscopy imaging by exploiting the phase difference of the fluorescence emission from different depths of the sample. The technique uses a modulated excitation beam and homodyne detection scheme. The image after homodyne detection consists of an unmodulated term and a sinusoidally modulated term. The unmodulated term does not have any phase dependence. The modulated term has a phase dependence on the depth of the sample from which emission is occurring. In the proposed methodology, the unmodulated term is removed giving rise to images with better depth perception.


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    Titel :

    Improvement in fluorescence confocal microscopy for obtaining better depth perception


    Beteiligte:
    Murukeshan, V.M (Autor:in) / Vaishakh, Manu (Autor:in) / Keey, Seah Leong (Autor:in)


    Erscheinungsdatum :

    2008-12-01


    Format / Umfang :

    367726 byte




    Medientyp :

    Aufsatz (Konferenz)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch




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