The Rapid Single Spore Enumeration Assay (RapidSSEA) is being developed for the Mars Technology Program to aid planetary protection personnel in their efforts to validate bioburden reduction on spacecraft surfaces prior to launch. RapidSSEA is based on imaging and counting individual bacterial spores in a microscope field-of-view, where the contrast is generated by a highly luminescent complex that forms when dipicolinic acid (DPA) is released from spores during germination and binds to terbium (Tb/sup 3+/) ions in the surrounding medium. Under pulsed UV excitation, the long-lived (/spl tau/ /spl sim/ 1 ms) DPA-triggered Tb luminescence is imaged by a lifetime-gated camera, essentially eliminating fluorescent interferents from the image, which enables counting of bacterial spores swabbed from environmental samples. Here we report results from the first side-by-side comparison of RapidSSEA and the NASA standard assay. Both assays were used to assess the spore population on stainless steel coupons inoculated with Bacillus atrophaeus spores. Using RapidSSEA, 38.0% /spl plusmn/ 5.8% of the total inoculated spore population was recovered and counted within 30 minutes.


    Zugriff

    Zugriff prüfen

    Verfügbarkeit in meiner Bibliothek prüfen

    Bestellung bei Subito €


    Exportieren, teilen und zitieren



    Titel :

    A Rapid Single Spore Enumeration Assay


    Beteiligte:
    Pun To Yung, (Autor:in) / Kempf, M.J. (Autor:in) / Ponce, A. (Autor:in)

    Erschienen in:

    Erscheinungsdatum :

    2006-01-01


    Format / Umfang :

    1063873 byte




    Medientyp :

    Aufsatz (Konferenz)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch



    AMP based sensitive and rapid spore detection assay

    Chen, F. / Suzuki, S. / Kulman, G. et al. | NTRS | 2004


    A sensitive real-time spore detection assay

    Chen, Fei / Suzuki, Shigeya / Kuhlman, Gregory et al. | NTRS | 2005



    Rapid Enumeration of Active Bacteria in Water After Disinfection

    Pyle, Barry H. / McFeters, Gordon A. / Lisle, John T. et al. | SAE Technical Papers | 1998


    Rapid micro-detection system for the enumeration of bacterial endospores

    Fei Chen, / Kazarians, G.A. / Venkateswaran, K. et al. | IEEE | 2006