Abstract Cultured human embryonic kidney cells were separated into electrophoretic subpopulations in laboratory experiments and in two separation experiments on the STS-8 (Challenger) Space Shuttle flight using the mid-deck Continuous Flow Electrophoretic Separator (CFES). Populations of cells from each fraction were cultured for the lifetime of the cells, and supernatant medium was withdrawn and replaced at 4-day intervals. Withdrawn medium was frozen at −120°C for subsequent analysis. Enzyme assays, antibodies and gel electrophoresis were used as analytical tools for the detection and quantitation of plasminogen activators in these samples. These assays of frozen culture supernatant fluids confirmed the electrophoretic separation of plasminogen-activator producing cells from non-producing cells, the isolation of cells capable of sustained production, and the separation of cells that produce different plasminogen activators from one another.


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    Titel :

    Further analyses of human kidney cell populations separated on the space shuttle


    Beteiligte:
    Stewart, Robin M. (Autor:in) / Todd, Paul (Autor:in) / Cole, Kenneth D. (Autor:in) / Morrison, Dennis R. (Autor:in)

    Erschienen in:

    Advances in Space Research ; 12 , 5 ; 223-229


    Erscheinungsdatum :

    01.01.1991


    Format / Umfang :

    7 pages




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch