AbstractWe studied the effect of random rotation on the expression of integrins in cultured astrocytes with or without the addition of divalent ions. Some cultures were immediately fixed, others were let to rest and then fixed. Cells were submitted to immunohistochemistry using antibodies to -tubulin, integrins, ATPase and caspase-7. Labeling of F-actin, TUNEL method and scanning and transmission electron microscopy were also performed. The results showed that within 1h of random rotation cytoskeleton was disorganized, cell division was impaired, and apoptosis were present. After 1h, expression of integrins was evident and enhanced by calcium addition. Recovering of cell organization occurred after 1h of rest. We may infer that prolonged modeled low G, a period of rest and addition of calcium ions stimulate integrins to cluster in focal contacts in order to have enough sticking capacity to adhere to the extra cellular matrix and allow internalization of divalent ions able to protect the inner compartment of the cell.
Low gravity and integrins in cultured glial cells
Acta Astronautica ; 63 , 7-10 ; 899-905
2008-04-25
7 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Low gravity and integrins in cultured glial cells
Online Contents | 2008
|Low gravity and integrins in cultured glial cells
Online Contents | 2008
|Low gravity and integrins in cultured glial cells
British Library Conference Proceedings | 2008
|Cultured stem cells are sensitive to gravity changes
Online Contents | 2008
|Cultured stem cells are sensitive to gravity changes
Elsevier | 2008
|