AbstractHigh-affinity Na+-dependent glutamate transporters of the plasma membrane mediate the glutamate uptake into neurons, and thus maintain low levels of extracellular glutamate in the synaptic cleft. The study focused on the release of glutamate by reversal of Na+-dependent glutamate transporters from rat brain nerve terminals (synaptosomes) under conditions of centrifuge-induced hypergravity. Flow cytometric analysis revealed similarity in the size and cytoplasmic granularity between synaptosomal preparations obtained from control and G-loaded animals (10G, 1h). The release of cytosolic l-[14C]glutamate from synaptosomes was evaluated using the protonophore FCCP, which dissipated synaptic vesicle proton gradient, thus synaptic vesicles were not able to keep glutamate inside and the latter enriched cytosol. FCCP per se induced the greater release of l-[14C]glutamate in hypergravity as compared to control (4.8±1.0% and 8.0±1.0% of total label). Exocytotic release of l-[14C]glutamate evoked by depolarization was reduced down to zero after FCCP application under both conditions studied. Depolarization stimulated release of cytosolic l-[14C]glutamate from synaptosomes preliminary treated with FCCP was considerably increased from 27.0±2.2% of total label in control to 35.0±2.3% in hypergravity. Non-transportable inhibitor of glutamate transporter dl-threo-β-benzyloxyaspartate was found to significantly inhibit high-KCl and FCCP-stimulated release of l-[14C]glutamate, confirming the release by reversal of glutamate transporters. The enhancement of transporter-mediated release of glutamate in hypergravity was found to result at least partially from the inhibition of the activity of Na/K-ATPase in the plasma membrane of synaptosomes. We suggested that hypergravity-induced alteration in transporter-mediated release of glutamate indicated hypoxic injury of neurons.


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    Titel :

    Presynaptic transporter-mediated release of glutamate evoked by the protonophore FCCP increases under altered gravity conditions


    Beteiligte:
    Borisova, T.A. (Autor:in) / Krisanova, N.V. (Autor:in)

    Erschienen in:

    Advances in Space Research ; 42 , 12 ; 1971-1979


    Erscheinungsdatum :

    2008-04-22


    Format / Umfang :

    9 pages




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch