Abstract Exposure of fungal conidia ( ) or spores of to extreme dryness or vacuum induces DNA lesions, including strand breaks and the formation of DNA-protein cross-links. In wet cells only a small amount of protein is bound to DNA, but exposure to conditions of lowered water activity results in an increasing number of cross-links between DNA and proteins. In fungal conidia these cross-links are detected after selective iodination (125J) of the DNA-bound proteins followed by gel electrophoresis and subsequent autoradiography. Another approach is the labelling of DNA with 32p by means of nick translation and the detection of differences in the electrophoretic mobility of DNA before and after digestion with proteinase K of proteins bound to DNA.
Extreme dryness and DNA-protein cross-links
Advances in Space Research ; 12 , 4 ; 265-270
1991-01-01
6 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
Survival in extreme dryness and DNA-single-strand breaks
Elsevier | 1991
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