AbstractOur scope was to study the effects of on ground random positioning machine rotation on swine testicular cells in culture. Cells of the 2 n karyotype line, from trypsinized swine testes, were submitted to modeled microgravity ( μ G ) using a 3D RPM from 15min to 24h. The cultured cells were then fixed and submitted to immunohistochemistry using antibodies to steroid dehydrogenases, heat shock proteins and the sodium pump ( Na + / K + ATPase).The results revealed that, after 15min at modeled μ G , all the cells showed damages at cytoskeletal level. The immunoreactions for 3 β HSD , 17 β HSD and Na + / K + ATPase were almost abolished. After 24h of treatment, the presence of the enzymes was restored, and small heat shock proteins were strongly immunostainable. On the other hand, in the 1 × G cultures, the expression of HSPs was very weak.We conclude that modeled μ G by random positioning of cells affects testicular cells in culture for a short time, while the normal activity of the cells is restored after 24h.


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    Titel :

    Effect of conditions of three dimensional clinostating on testicular cell machinery


    Beteiligte:

    Erschienen in:

    Acta Astronautica ; 60 , 4-7 ; 391-396


    Erscheinungsdatum :

    2006-01-01


    Format / Umfang :

    6 pages




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch





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