Abstract DNA damage induced by heavy ions in bacterial cells and bacteriophages such as Bacillus subtilis, E. coli and Bacteriophage Tl were investigated by analyzing the double strand breaks in the chromosomal DNA. This kind of lesion is considered as one of the main reasons for lethal events. To analyze double strand breaks in long molecules of DNA - up to some Mbp in length - the technique of pulse field agarose gel electrophoresis has been used. This allows the detection of one double strand break per genome. Cell lysis and DNA isolation were performed in small agarose blocks directly. This procedure secured minimum DNA destruction by shearing forces. After running a gel, the DNA was stained with ethidium bromide. The light intensity of ethidium bromide fluorescence for both the outcoming (running) DNA and the remaining intact DNA were measured by scanning. The mean number of double strand breaks was calculated by determining the quotient of these intensities. Strand break induction after heavy ion and X-ray irradiation was compared.


    Zugriff

    Zugriff prüfen

    Verfügbarkeit in meiner Bibliothek prüfen

    Bestellung bei Subito €


    Exportieren, teilen und zitieren



    Titel :

    Heavy ion induced double strand breaks in bacteria and bacteriophages


    Beteiligte:
    Micke, U. (Autor:in) / Schäfer, M. (Autor:in) / Anton, A. (Autor:in) / Horneck, G. (Autor:in) / Bücker, H. (Autor:in)

    Erschienen in:

    Advances in Space Research ; 12 , 2-3 ; 59-63


    Erscheinungsdatum :

    1991-01-01


    Format / Umfang :

    5 pages




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch



    Heavy ion induced DNA double strand breaks in cells of E. coli

    Schäfer, M. / Schmitz, C. / Bücker, H. | Elsevier | 1994





    Strand Breaks in DNA Samples Induced with LASERIX

    Zielbauer, B. / Habib, J. / Kazamias, S. et al. | Springer Verlag | 2009