A microfabricated magnetoelastic sensor immobilized with specific phage for the detection of Bacillus anthracis spores is presented. This micro-sensor can detect response signal remotely through magnetic field, enabling the in-situ measurement of target pathogens in aqueous environment. The sensor's surface was modified by selected phage which provides the binding ability to B. anthracis spores. When exposed to B. anthracis spore solution, the resonant frequency of the sensor decreased due to the accumulated spore attachment on the surface. Sensors with dimensions of 200 x 40 x 4 micrometer were employed in the tests of increasing concentrations (102 to 108 cfu/ml) of B. anthracis spores. A detection limit of 102 cfu/ml, and a sensitivity of 13.1 kHz/decade, were observed. Scanning electron microscopy (SEM) photographs demonstrated binding of target analytes to biosensors compared to controls.


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    Titel :

    Characterization of phage-coupled magnetoelastic micro-particles for the detection of Bacillus anthracis Sterne spores


    Beteiligte:
    Wan, Jiehui (Autor:in) / Johnson, M.L. (Autor:in) / Horikawa, S. (Autor:in) / Chin, B.A. (Autor:in) / Petrenko, V.A. (Autor:in)

    Erschienen in:

    Erscheinungsdatum :

    2007


    Format / Umfang :

    4 Seiten, 17 Quellen




    Medientyp :

    Aufsatz (Konferenz)


    Format :

    Print


    Sprache :

    Englisch




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