A multimodal multiphoton microscopy (MPM) is developed to acquire two-photon and three-photon signals simultaneously, including two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and third harmonic generation (THG). We have developed a miniature multimodal MPM system based on a dual-wavelength Erdoped fiber laser, which includes the fundamental pulse at 1580 nm to excite THG and the frequency-doubled pulse at 790 nm to excite TPEF and SHG. The laser is coupled by a single mode fiber into a miniature MPM imaging probe. Label-free imaging by TPEF, SHG, and THG are demonstrated on biological samples, obtained from intrinsic fluorophores, collagen, lipid and interface.
Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser
Multiphoton Microscopy in the Biomedical Sciences XIX ; 2019 ; San Francisco,California,United States
Proc. SPIE ; 10882
2019-02-22
Aufsatz (Konferenz)
Elektronische Ressource
Englisch
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