A multimodal multiphoton microscopy (MPM) is developed to acquire two-photon and three-photon signals simultaneously, including two-photon excitation fluorescence (TPEF), second harmonic generation (SHG), and third harmonic generation (THG). We have developed a miniature multimodal MPM system based on a dual-wavelength Erdoped fiber laser, which includes the fundamental pulse at 1580 nm to excite THG and the frequency-doubled pulse at 790 nm to excite TPEF and SHG. The laser is coupled by a single mode fiber into a miniature MPM imaging probe. Label-free imaging by TPEF, SHG, and THG are demonstrated on biological samples, obtained from intrinsic fluorophores, collagen, lipid and interface.


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    Titel :

    Simultaneous two-photon and three-photon microscopy imaging with a dual-wavelength Er-doped fiber laser


    Beteiligte:
    Huang, Lin (Autor:in) / Tang, Shuo (Autor:in)

    Kongress:

    Multiphoton Microscopy in the Biomedical Sciences XIX ; 2019 ; San Francisco,California,United States


    Erschienen in:

    Proc. SPIE ; 10882


    Erscheinungsdatum :

    2019-02-22





    Medientyp :

    Aufsatz (Konferenz)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch




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