The nematode Caenorhabditis elegans was exposed to natural space radiation using the ESA biorack facility aboard Spacelab on International Microgravity Laboratory 1, STS-42. For the major experimental objective dormant animals were suspended in buffer or on agar or immobilized next to CR-39 plastic nuclear track detectors to correlate fluence of HZE particles with genetic events. This configuration was used to isolate mutations in a set of 350 essential genes as well as in the unc-22 structural gene. From flight samples 13 mutants in the unc-22 gene were isolated along with 53 lethal mutations from autosomal regions balanced by a translocation eT1(III;V). Preliminary analysis suggests that mutants from worms correlated with specific cosmic ray tracks may have a higher proportion of rearrangements than those isolated from tube cultures on a randomly sampled basis. Flight sample mutation rate was approximately 8-fold higher than ground controls which exhibited laboratory spontaneous frequencies.


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    Titel :

    Radiation effects in nematodes: Results from IML-1 experiments


    Beteiligte:
    Nelson, G. A. (Autor:in) / Schubert, W. W. (Autor:in) / Kazarians, G. A. (Autor:in) / Richards, G. F. (Autor:in) / Benton, E. V. (Autor:in) / Benton, E. R. (Autor:in) / Henke, R. (Autor:in)


    Erscheinungsdatum :

    1994-10-01



    Medientyp :

    Sonstige


    Format :

    Keine Angabe


    Sprache :

    Englisch




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    Nelson, G.A. / Schubert, W.W. / Kazarians, G.A. et al. | Elsevier | 1994



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    Development and Chromosome Mechanics in Nematodes: Results from IML-1

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    Development and chromosome mechanics in nematodes: Results from IML-1

    Nelson, G. A. / Schubert, W. W. / Kazarians, G. A. et al. | NTRS | 1994