AbstractIsolates from the Mir space station identified as Pseudomonas sp. and Stenotrophomonas maltophilia were subjected to clinorotation to model reduced gravity conditions in water in slow turning lateral vessels (STLVs). To examine cells in varying physiological states, bacteria were enumerated based on the Live/Dead BacLight kit, DAPI (4′,6-diamidino-2-phenylindole) staining, fluorescent in situ hybridization (FISH), and colony forming units (CFU). Both Pseudomonas sp. and S. maltophilia showed a slight increase in abundance over time but only cells of Pseudomonas sp. were affected by modeled reduced gravity. For Pseudomonas sp. numbers of DAPI stained cells were significantly higher under modeled reduced gravity compared to normal gravity. In addition, the abundance of cells attached to stainless steel disks, on one sampling date, was greater for the Pseudomonas isolate under modeled reduced gravity than normal gravity. The isolates examined did not appear to appreciably enter into a viable, but not culturable state during the experiments. In general, differences between treatments were not great, demonstrating that responses to reduced gravity are less apparent under starvation conditions, compared to earlier studies which used more rich nutrient sources.


    Zugriff

    Zugriff prüfen

    Verfügbarkeit in meiner Bibliothek prüfen

    Bestellung bei Subito €


    Exportieren, teilen und zitieren



    Titel :

    Mir space station bacteria responses to modeled reduced gravity under starvation conditions


    Beteiligte:
    Baker, Paul W. (Autor:in) / Leff, Laura G. (Autor:in)

    Erschienen in:

    Advances in Space Research ; 38 , 6 ; 1152-1158


    Erscheinungsdatum :

    2006-05-18


    Format / Umfang :

    7 pages




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch





    Survival of Pathogenic Bacteria Under Nutrient Starvation Conditions

    Maki, James / Boyle, Michael / Mitchell, Ralph et al. | SAE Technical Papers | 1990


    Meniscus shape under reduced-gravity conditions.

    Chin, J. H. / Satterlee, H. M. | NTRS | 1965


    Gas-Liquid Jets under Reduced Gravity Conditions

    Pampern, O. / Rath, H. J. | British Library Online Contents | 1992


    Saltation threshold experiments conducted under reduced gravity conditions

    WHITE, B. / GREELEY, R. / LEACH, R. et al. | AIAA | 1987