Abstract The purpose of this experiment was to estimate the protective effects of melatonin against radiation-induced brain damages in mice induced by heavy ion beams. Kun-Ming mice were randomly divided into five groups: normal control group, irradiation control group, and three different doses of melatonin (5, 10, and 20mg/kg, i.p.) treated groups. Apart from the normal control group, the other four groups were exposed to whole-body 4.0Gy carbon ion beam irradiation (approximately 0.5Gy/min) after i.p. administration of normal saline or melatonin 1h before irradiation. The oxidative redox status of brain tissue was assessed by measurement of malondiadehyde (MDA) levels, total superoxide dismutase (T-SOD), cytosolic superoxide dismutase (Cu/ZnSOD, SOD1) and mitochondrial superoxide dismutase (MnSOD, SOD2) activities at 8h after irradiation. DNA damages were determined using the Comet assay and apoptosis and cell cycle distribution were detected by flow cytometric analyses. A dramatic dose-dependent decrease in MDA levels, tail moment, rates of tailing cells, and apoptosis, and a dose-dependent increase in T-SOD and SOD2 activities, in brain tissues in the melatonin-treated groups were detected compared with the irradiation only group. Furthermore, flow cytometric analysis demonstrated that the percentage of brain cells in the G0/G1 phase decreased significantly, while those in the S and G2/M stage increased dramatically, with mice pretreated with melatonin compared to the irradiation control group. These data indicate that melatonin has protective effects against irradiation-induced brain injury, and that its underlying protective mechanisms may relate to modulation of oxidative stress induced by heavy ionirradiation.


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    Titel :

    Protective effects of melatonin against 12C6+ beam irradiation-induced oxidative stress and DNA injury in the mouse brain


    Beteiligte:
    Wu, Z.H. (Autor:in) / Zhang, H. (Autor:in) / Wang, X.Y. (Autor:in) / Yang, R. (Autor:in) / Liu, B. (Autor:in) / Liu, Y. (Autor:in) / Zhao, W.P. (Autor:in) / Feng, H.Y. (Autor:in) / Xue, L.G. (Autor:in) / Hao, J.F. (Autor:in)

    Erschienen in:

    Advances in Space Research ; 49 , 1 ; 196-203


    Erscheinungsdatum :

    2011-09-12


    Format / Umfang :

    8 pages




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch