Highlights ► We isolated ten simulated microgravity stress (SMS) inducible genes from seedlings and mature plants of Arabidopsis. ► WRKY46, a unique plant transcription factor was specifically induced by SMS, but not by other abiotic stresses. ► WRKY46 including other SMS inducible genes has a spatial arrangement of cis-elements on promoter region. ► WRKY46 can be a novel SMS transcription factor.

    Abstract Arabidopsis gene expression microarray (44K) was used to detect genes highly induced under simulated microgravity stress (SMS). Ten SMS-inducible genes were selected from the microarray data and these 10 genes were found to be abundantly expressed in 3-week-old plants. Nine out of the 10 SMS-inducible genes were also expressed in response to the three abiotic stresses of drought, touch, and wounding in 3-week-old Arabidopsis plants respectively. However, WRKY46 was elevated only in response to SMS. Six other WRKY genes did not respond to SMS. To clarify the characteristics of the genes expressed at high levels in response to SMS, 20 cis-elements in the promoters of the 40 selected genes including the 10 SMS-inducible genes, the 6 WRKY genes, and abiotic stress-inducible genes were analyzed and their spatial positions on each promoter were determined. Four cis-elements (M/T-G-T-P from MYB1AT or TATABOX5, GT1CONSENSUS, TATABOX5, and POLASIG1) showed a unique spatial arrangement in most SMS-inducible genes including WRKY46. Therefore the M/T-G-T-P cis-element patterns identified in the promoter of WRKY46 may play important roles in regulating gene expression in response to SMS. The presences of the cis-element patterns suggest that the order or spatial positioning of certain groups of cis-elements is more important than the existence or numbers of specific cis-elements. Taken together, our data indicate that WRKY46 is a novel SMS inducible transcription factor and the unique spatial arrangement of cis-elements shown in WRKY46 promoter may play an important role for its response to SMS.


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    Titel :

    Identification of unique cis-element pattern on simulated microgravity treated Arabidopsis by in silico and gene expression


    Beteiligte:
    Soh, Hyuncheol (Autor:in) / Choi, Yongsang (Autor:in) / Lee, Taek-Kyun (Autor:in) / Yeo, Up-Dong (Autor:in) / Han, Kyeongsik (Autor:in) / Auh, Chungkyun (Autor:in) / Lee, Sukchan (Autor:in)

    Erschienen in:

    Advances in Space Research ; 50 , 3 ; 397-407


    Erscheinungsdatum :

    2012-04-23


    Format / Umfang :

    11 pages




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch