AbstractAcute exposure of mammalian cells to ionizing radiation results in a delay of cell-cycle progression and/or augmentation of apoptosis. Following ionizing radiation-induced DNA damage, cell-cycle arrest in the G1- or G2-phase of the cell-cycle prevents or delays DNA replication or mitosis, providing time for the DNA repair machinery to exert its function. Deregulation or failing of cell-cycle checkpoints and/or DNA repair mechanisms may lead normal cells bearing chromosome mutations to acquire neoplastic autonomy, which in turn can trigger the onset of cancer. Existing studies have focused on the impact of p53 status on the radiation response of lung cancer (LC) cell lines in terms of both cell-cycle regulation and apoptosis, while no comparative studies have been performed on the radiation response of lung derived normal and cancerous epithelial cells. To investigate the radiation response in normal and cancerous phenotypes, along with the role and impact of p53 status, and possible correlations with pRb/p105 or other proteins involved in carcinogenesis and cell-cycle regulation, we selected two lung-derived epithelial cell lines, one normal (NL20, p53 wild-type) and one non-small cell lung cancer (NSCLC), H358 (known to be p53-deficient). We compared the levels of γ-induced cell proliferation ability, cell-cycle arrest, apoptotic index, and expression levels of cell-cycle regulating and regulated proteins. The different cell sensitivity, apoptotic response and protein expression profiles resulting from our study for NL20 and H358 cells suggest that still unknown mechanisms involving p53, pRb/p105 and their target molecules might play a pivotal role in determining cell sensitivity and resistance upon exposure to ionizing radiation.


    Zugriff

    Zugriff prüfen

    Verfügbarkeit in meiner Bibliothek prüfen

    Bestellung bei Subito €


    Exportieren, teilen und zitieren



    Titel :

    Analysis of cell-cycle regulation following exposure of lung-derived cells to γ-rays


    Beteiligte:
    Trani, D. (Autor:in) / Lucchetti, C. (Autor:in) / Cassone, M. (Autor:in) / D’Agostino, L. (Autor:in) / Caputi, M. (Autor:in) / Giordano, A. (Autor:in)

    Erschienen in:

    Advances in Space Research ; 39 , 6 ; 1026-1033


    Erscheinungsdatum :

    2006-11-15


    Format / Umfang :

    8 pages




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch





    Simultaneous Exposure of Mammalian Cells to Heavy Ions and X-Rays

    Furusawa, Y. / Aoki, M. / Durante, M. et al. | British Library Conference Proceedings | 2002


    Rays of Risk: Air Travel and Radiation Exposure

    Kahn, F. / Southern California Safety Institute | British Library Conference Proceedings | 2005


    Regulation of cell cycle arrest by the Bhlh protein Mtwist

    Spicer, D. B. / Cheung, W. / Lassar, A. B. et al. | British Library Conference Proceedings | 1997