Highlights ► Simulated weightlessness disrupts microfilaments in osteoblasts. ► Simulated weightlessness inhibits the osteoblastic proliferation. ► Simulated weightlessness inhibits the migration of osteoblasts. ► Simulated weightlessness induces Ca2+ influx through stretch-activated channels. ► Ca2+ influx causes microfilament disruption induced by simulated weightlessness.

    Abstract We have explored the role of Ca2+ signaling in microfilament reorganization of osteoblasts induced by simulated weightlessness using a random positioning machine (RPM). The RPM-induced alterations of cell morphology, microfilament distribution, cell proliferation, cell migration, cytosol free calcium concentration ([Ca2+]i), and protein expression in MG63 osteoblasts were investigated. Simulated weightlessness reduced cell size, disrupted microfilament, inhibited cellular proliferation and migration, and induced an increase in [Ca2+]i in MG63 human osteosarcoma cells. Gadolinium chloride (Gd), an inhibitor for stretch-activated channels, attenuated the increase in [Ca2+]i and microfilament disruption. Further, the expression of calmodulin was significantly increased by simulated weightlessness, and an inhibitor of calmodulin, W-7, aggravated microfilament disruption. Our findings demonstrate that simulated weightlessness induces Ca2+ influx through stretch-activated channels, then results in microfilament disruption.


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    Titel :

    Calcium influx through stretch-activated channels mediates microfilament reorganization in osteoblasts under simulated weightlessness


    Beteiligte:
    Luo, Mingzhi (Autor:in) / Yang, Zhouqi (Autor:in) / Li, Jingbao (Autor:in) / Xu, Huiyun (Autor:in) / Li, Shengsheng (Autor:in) / Zhang, Wei (Autor:in) / Qian, Airong (Autor:in) / Shang, Peng (Autor:in)

    Erschienen in:

    Advances in Space Research ; 51 , 11 ; 2058-2068


    Erscheinungsdatum :

    2013-01-07


    Format / Umfang :

    11 pages




    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch





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