Highlights ► Simulated weightlessness disrupts microfilaments in osteoblasts. ► Simulated weightlessness inhibits the osteoblastic proliferation. ► Simulated weightlessness inhibits the migration of osteoblasts. ► Simulated weightlessness induces Ca2+ influx through stretch-activated channels. ► Ca2+ influx causes microfilament disruption induced by simulated weightlessness.
Abstract We have explored the role of Ca2+ signaling in microfilament reorganization of osteoblasts induced by simulated weightlessness using a random positioning machine (RPM). The RPM-induced alterations of cell morphology, microfilament distribution, cell proliferation, cell migration, cytosol free calcium concentration ([Ca2+]i), and protein expression in MG63 osteoblasts were investigated. Simulated weightlessness reduced cell size, disrupted microfilament, inhibited cellular proliferation and migration, and induced an increase in [Ca2+]i in MG63 human osteosarcoma cells. Gadolinium chloride (Gd), an inhibitor for stretch-activated channels, attenuated the increase in [Ca2+]i and microfilament disruption. Further, the expression of calmodulin was significantly increased by simulated weightlessness, and an inhibitor of calmodulin, W-7, aggravated microfilament disruption. Our findings demonstrate that simulated weightlessness induces Ca2+ influx through stretch-activated channels, then results in microfilament disruption.
Calcium influx through stretch-activated channels mediates microfilament reorganization in osteoblasts under simulated weightlessness
Advances in Space Research ; 51 , 11 ; 2058-2068
2013-01-07
11 pages
Aufsatz (Zeitschrift)
Elektronische Ressource
Englisch
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