Filtration through membranes with nanopores is typically associated with high transmembrane pressures and high energy consumption. This problem can be addressed by reducing the respective membrane thickness, and the present thesis describes a simple procedure to prepare ultrathin membranes based on protein nanopores. To form such membranes, the transmembrane protein ferric hydroxamate uptake protein component A (FhuA) or its open-pore variant are assembled at the air-water interface of a Langmuir trough, compressed to a dense film, crosslinked by glutaraldehyde, and transferred to various support materials. This approach allows to prepare monolayer or multilayer membranes with a very high density of protein nanopores aligned vertically to the membrane plane, as demonstrated by sum-frequency generation (SFG) spectroscopy. Freestanding membranes covering holes up to 7 µm in diameter are visualized by atomic force microscopy (AFM), helium ion microscopy (HIM), and transmission electron microscopy (TEM). AFM PeakForce quantitative nanomechanical property mapping (PeakForce QNM) demonstrates remarkable mechanical stability and elastic properties of freestanding monolayer membranes with a thickness of only 5 nm. Multilayer membranes significantly reject bovine serum albumin (BSA) molecules with a molecular size below 10 nm, and exhibit excellent water permeance, two orders of magnitude superior to comparable, industrially applied membranes. Furthermore, incorporation of either closed or open protein nanopores allows tailoring the membrane’s ion permeability. In addition, first attempts to similar membrane formation with nanopores based on tobacco mosaic virus (TMV) discs could be demonstrated. The new protein membrane could pave the way to energy-efficient nanofiltration.


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    Titel :

    Protein nanopore membranes prepared by a simple Langmuir-Schaefer approach


    Weitere Titelangaben:

    Ein einfacher Langmuir-Schäfer-Ansatz zur Herstellung von Protein-Nanoporen-Membranen


    Beteiligte:

    Erscheinungsdatum :

    2021



    Medientyp :

    Sonstige


    Format :

    Elektronische Ressource


    Sprache :

    Englisch



    Klassifikation :

    DDC:    629




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