Svrha rada: Usporediti citotoksičnost/genotoksičnost zagrijanih kompozita polimeriziranih preko CAD/CAM overleja na kulturi izoliranih ljudskih limfocita. Materijali i postupci: Mikrohibridni (Z100, 3M ESPE) i nanopunjeni kompozit (Filtek Supreme Ultra, 3M ESPE) zagrijani su u uređaju za zagrijavanje kompozita (Calset, AdDent Inc.) na temperaturama od 37 °C (T1), 54 °C (T2) i 68 °C (T3). Mala količina zagrijanog kompozita stavljena je u cilindrični kalup (promjera 6 mm, debljine 0.65 mm), prekrivena Mylar folijom, sprešana, te osvjetljena izravno, preko CAD/CAM keramički pojačanog polimera (CRP) (LAVA Ultimate, 3M ESPE) ili CAD/CAM litij disilikatnog keramičkog overlaja (LDC)(e.max, Ivoclar/Vivadent) debljine 2 mm. Odmah nakon osvjetljavanja uzorci su stavljeni u staničnu kulturu limfocita izoliranih iz periferne krvi. Citotoksičnost je izmjerena metodom dvojnog bojenja etidijevim bromidom i akridinskom narančastom bojom koja omogućuje određivanje postotka živih stanica te stanica u apoptozi i nekrozi na osnovi njihovih morfoloških obilježja. Genotoksičnost je procijenjena uporabom komet-testa u alkalnim uvjetima. Rezultati: Za Z100, najveći postotak živih stanica zabilježen je na T1 (93,7 %) nakon izravnog osvjetljavanja; slijedi osvjetljavanje preko CRP-a (92,3 %) te LDC-a (91,7 % T1, T3). Za Filtek Supreme Ultra najveći postotak živih stranica zabilježen je nakon osvjetljavanja preko CRP-a (91,2 % T2), LDC-a (90 % T1, T3) te pri izravnom osvjetljavanju (88,7 % T2). Zaključak: Za oba ispitivana materijala, zagrijavanje na T1 i T2 postupak je izbora. S obzirom na genotoksičnost, ne preporučuje se zagrijavanje na T3. ; Objectives: The aim was to compare cytotoxicity/genotoxicity of pre-heated composites polymerized through CAD/CAM overlays on isolated human peripheral blood lymphocytes. Material and Methods: A microhybrid (Z100, 3M ESPE) and nanofilled composite (Filtek Supreme Ultra, 3M ESPE) were heated in a heating unit (Calset, AdDent Inc.) at different temperatures: 37 oC, 54 oC, and 68 oC. A small amount of heated composite was placed in a cylindrical mold (6mm diameter; 0.65mm thick), covered with a Mylar sheet, pressed and light-cured directly and through 2 mm thick CAD/CAM ceramic-reinforced polymer (CRP)(LAVA Ultimate, 3M ESPE) or CAD/CAM lithium disilicate ceramic (LDC)(e.max, Ivoclar/Vivadent) overlay. After curing, the specimens were immediately placed in a prepared lymphocyte cell culture. Cytotoxicity was assessed using a dye exclusion method by simultaneous staining with ethidium bromide and acridine orange, aimed to determine percentages of viable, apoptotic and necrotic cells. Genotoxicity was studied using alkaline comet assay. Results: For Z100, the highest percentage of viable cells is recorded at T1 (93.7%) after direct light curing, followed by light curing through CRP (92.3%) and through LDC (91.7%T1,T3). For Filtek Supreme Ultra, the highest percentage of viable cells is recorded while curing through CRP (91.0% T2), followed by LDC (90% T1,T3) and direct light curing (88.7%T2). Conclusion: For both tested materials, preheating the procedure at T1 and T2 may be the procedure of choice. In terms of genotoxicity, reheating at T3 may not be suggested.


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    Titel :

    Toksiksičnost prethodno zagrijanih kompozita izravnom polimerizacijom i preko CAD / CAM overleja ; Toxicity of Pre-heated Composites Polymerized Directly and Through CAD/CAM Overlay


    Beteiligte:
    Knezevic, Alena (Autor:in) / Zeljezic, Davor (Autor:in) / Kopjar, Nevenka (Autor:in) / Duarte, Jr., Sillas (Autor:in) / Par, Matej (Autor:in) / Tarle, Zrinka (Autor:in)

    Erscheinungsdatum :

    2018-09-17


    Anmerkungen:

    Acta stomatologica Croatica ; ISSN 0001-7019 (Print) ; ISSN 1846-0410 (Online) ; Volume 52 ; Issue 3


    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch , Kroatisch



    Klassifikation :

    DDC:    629





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