Abstract BACKGROUND: In the rat, the single-pellet reaching task includes orienting, reaching, grasping and retracting movements. It has previously been described by notation techniques, high-speed video and cineradiographic recordings. Recently, high-definition cameras have been used to track paw and digit movements with DeepLabCut, a machine-learning algorithm for markerless estimation of paw position. NEW METHOD: Our new approach consists of positioning three high-speed infrared digital cameras to track the full motion of markers on the rat's body. This provided a previously unavailable 3D recording of skilled reaching kinematics in the rat moving freely in the reaching box, which were analysed by Qualisys Track Manager software and MATLAB. RESULTS: This method enabled description of kinematic parameters unobtainable without motion tracking and provided insight into the spatiotemporal metrics of movements used to perform skilled reaching. It revealed that orientation features three steps and reaching has two bimodal start-point distributions, one along the horizontal axis and one along the vertical axis. At the end of reaching, the wrist/paw occupies the same position as the nose at the end of orienting. In grasping, averaging trajectories confirmed the marker lowering and target approaching. COMPARISON WITH EXISTING METHODS: Our method required significantly reduced time to label data and obviates the need for off-line manual marking of videos. It provides an efficient means of capturing volumes containing the entire range of marker movements. CONCLUSIONS: This study validated a new and efficient approach for quantifying rat movement kinematics, useful for comparing preclinical and clinical conditions.


    Zugriff

    Download


    Exportieren, teilen und zitieren



    Titel :

    A kinematic study of skilled reaching movement in rat



    Erscheinungsdatum :

    2019-01-01



    Medientyp :

    Aufsatz (Zeitschrift)


    Format :

    Elektronische Ressource


    Sprache :

    Englisch



    Klassifikation :

    DDC:    629




    Reaching to inhibit a prepotent response: a wearable 3-axis accelerometer kinematic analysis

    Alessia Angeli / Irene Valori / Teresa Farroni et al. | BASE | 2021

    Freier Zugriff

    Reaching to inhibit a prepotent response: A wearable 3-axis accelerometer kinematic analysis

    Angeli A. / Valori I. / Farroni T. et al. | BASE | 2021

    Freier Zugriff

    Statistical Analysis and Kinematic Assessment of Upper Limb Reaching Task in Parkinson’s Disease

    Ponsiglione A. M. / Ricciardi C. / Amato F. et al. | BASE | 2022

    Freier Zugriff

    Study on the Effects of Motorcycle Anti-Lock-Braking-System for Skilled and Less-Skilled Riders

    Toyofuku, Y. / Matsushima, K. / Irie, Y. et al. | British Library Conference Proceedings | 1993