Bioactive glasses (BGs) continue to attract interest as promising materials for bone regeneration. In the broad family of BGs, boron containing systems, including B-doped silicate, borosilicate glasses and borate glasses, are being increasingly considered due to their attractive osteogenic and neovascularization properties. In this work, a new series of BGs based on the 13-93 composition (SiO2-CaO-Na2O-P2O5-K2O-MgO-B2O3) was prepared and three-dimensional scaffolds were produced by the polymer replica technique. The compressive strength of these scaffolds was found to be significantly higher than that of standard 45S5 Bioglass® scaffolds which is related to the higher densification rate of struts achieved by viscous flow during the sintering process. In vitro dissolution studies in cell culture medium were carried out over a period of 14 days and the formation of hydroxyapatite (HA) was confirmed using different characterization techniques such as Scanning Electron Microscopy (SEM), X-Ray Diffraction (XRD) and Fourier Transform Infrared Spectroscopy (FTIR). The boron-based scaffolds immersed in cell culture medium exhibited a moderate release of B and Si ions which was measured using Inductively Coupled Plasma-Optical emission analyses. The effects of the ionic dissolution products (IDPs) of the boron-based BG scaffolds on mouse bone marrow stromal cells (ST2 cells) in vitro were ascertained, using an angiogenesis assay. The expression and secretion of angiogenic growth factor, vascular endothelial growth factor (VEGF) from ST2 cells were measured quantitatively using the VEGF ELISA Kit. Cellular response was found to be dependent on the boron content and the B release profile from the glasses corresponded to the positive/negative influence in their biological activity. Functionalizing the surfaces of these BG scaffolds with biologically active molecules or enzymes is a suitable strategy to enhance both the inorganic and biological responses of the biomaterial and to stimulate physiological tissue regeneration. ...


    Zugriff

    Download


    Exportieren, teilen und zitieren



    Titel :

    Surface Modification of Bioactive Glass Scaffolds ; Oberflächenmodifikation von bioaktiven Glasstützstrukturen


    Beteiligte:

    Erscheinungsdatum :

    2017-01-01


    Medientyp :

    Hochschulschrift


    Format :

    Elektronische Ressource


    Sprache :

    Englisch



    Klassifikation :

    DDC:    629